4FMP
Crystal structure of thermostable, organic-solvent tolerant lipase from Geobacillus sp. strain ARM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.786, 143.392, 63.965 |
| Unit cell angles | 90.00, 105.88, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.17627 |
| Rwork | 0.173 |
| R-free | 0.24165 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dsn |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.854 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 1.480 | 2.300 |
| Rmerge | 0.114 | |
| Number of reflections | 42814 | |
| <I/σ(I)> | 12.5 | 2 |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 293 | 0.1M MES monohydrate, 12% v/v PEG 20000, pH 6.5, EVAPORATION, temperature 293K |
