4FMJ
Merkel cell polyomavirus VP1 in complex with GD1a oligosaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2010-10-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.660, 130.150, 165.530 |
| Unit cell angles | 98.04, 101.02, 105.81 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.19297 |
| Rwork | 0.192 |
| R-free | 0.24623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.101 | 0.666 |
| Number of reflections | 248679 | |
| <I/σ(I)> | 8.74 | 2.07 |
| Completeness [%] | 97.3 | 96.8 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M sodium cacodylate, pH 6.5, 6% w/v PEG3350, 0.3 M magnesium chloride, soaked in 25 mM GD1a oligosaccharide, cryoprotection: 25% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
