4FLN

Crystal structure of plant protease Deg2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Detector technology	CCD
Collection date	2011-09-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	125.398, 188.165, 166.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.742 - 2.800
R-factor	0.2071
Rwork	0.203
R-free	0.27420
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.224
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.104	0.435
Number of reflections	48456
<I/σ(I)>	14.8	4.2
Completeness [%]	98.9	99.3
Redundancy	5.2	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1