4FLK
Human MetAP1 with bengamide analog Y10, in Mn form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.777, 77.506, 48.620 |
| Unit cell angles | 90.00, 90.43, 90.00 |
Refinement procedure
| Resolution | 31.290 - 1.470 |
| R-factor | 0.1999 |
| Rwork | 0.199 |
| R-free | 0.22080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.430 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.500 |
| High resolution limit [Å] | 1.470 | 3.990 | 1.470 |
| Rmerge | 0.095 | 0.068 | 0.385 |
| Number of reflections | 60021 | ||
| <I/σ(I)> | 17.8 | ||
| Completeness [%] | 99.5 | 96.1 | 96.1 |
| Redundancy | 3.6 | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 7.2 | 200 mM sodium formate, 20% PEG 3350, pH 7.2, microbatch under oil |
