4FLA

Crystal structure of human RPRD1B, carboxy-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Detector technology	CCD
Collection date	2012-02-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97931
Spacegroup name	P 4 21 2
Unit cell lengths	100.221, 100.221, 142.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.200
R-factor	0.233
Rwork	0.232
R-free	0.25750
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.311
Data reduction software	XDS
Data scaling software	SCALA (CCP4_3.3.20)
Phasing software	SHELX
Refinement software	REFMAC (5.7.0027)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.630	47.630	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge	0.116	0.020	0.930
Total number of observations		11361	52846
Number of reflections	37701
<I/σ(I)>	15.5351
Completeness [%]	100.0	99.59	100
Redundancy	9.62	8.41	9.81

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	25% PEG-3350, 0.2M ammonium acetate, 0.1M HEPES, pH 7.5, vapor diffusion, temperature 291K
1	VAPOR DIFFUSION	7.5	291	25% PEG-3350, 0.2M ammonium acetate, 0.1M HEPES, pH 7.5, vapor diffusion, temperature 291K