4FK9

High Resolution Structure of the Catalytic Domain of Mannanase SActE_2347 from Streptomyces sp. SirexAA-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-04-18
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97857
Spacegroup name	P 21 21 2
Unit cell lengths	62.805, 102.360, 45.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.765 - 1.060
R-factor	0.1187
Rwork	0.118
R-free	0.13150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.232
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1061)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.080
High resolution limit [Å]	1.060	2.880	1.060
Rmerge	0.063	0.048	0.315
Number of reflections	130004
<I/σ(I)>	12.8
Completeness [%]	97.7	90.3	95.3
Redundancy	3.5	3.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1