4FK8

Crystal structure of FERREDOXIN-NADP REDUCTASE from burkholderia thailandensis E264 with bound FAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-05
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	70.580, 86.200, 102.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.940 - 2.100
R-factor	0.199
Rwork	0.198
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.404
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.081	0.473
Number of reflections	36808
<I/σ(I)>	18.59	4.3
Completeness [%]	99.3	92.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7		EBS INTERNAL TRACKING NUMBER 229491d3 HEPES (PH 7.0), 500 MM NACL, 2 MM DTT, 0.025% SODIUM AZIDE, 5% GLYCEROL, 0.4 UL X 0.4 UL DROP WITH 20% PEG 3350, 200 MM NACL. SOAKED FOR 5 DAYS WITH 1 MM FAD. 20% ETHYLENE GLYCOL used as CRYOPROTECTANT, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 289K