4FJX

RB69 DNA polymerase ternary complex with dATP/dG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	110
Detector technology	CCD
Collection date	2011-10-15
Detector	APEX II CCD
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	75.052, 119.860, 130.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.150 - 2.110
R-factor	0.18535
Rwork	0.183
R-free	0.22518
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.062
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.110	2.110
Number of reflections	63686
Completeness [%]	94.9	94.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5 , VAPOR DIFFUSION, temperature 298K