4FJS

Crystal structure of ureidoglycolate dehydrogenase enzyme in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-26
Detector	ADSC QUANTUM 315r
Spacegroup name	P 42 21 2
Unit cell lengths	162.760, 162.760, 61.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.180 - 2.130
R-factor	0.223
Rwork	0.222
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1XHR
RMSD bond length	0.009
RMSD bond angle	1.113
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	1.700	2.500
Rmerge	0.123	0.606
Number of reflections	46352
<I/σ(I)>	6.7
Completeness [%]	99.9	99.9
Redundancy	23.2	16.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6		0.1M MES, 4.0M NACL, pH 6.0, EVAPORATION