4FJR
Mode of interaction of Merocyanine 540 with HEW Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-12-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.770, 78.770, 37.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.690 - 1.860 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.091 | 0.328 |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 98.6 | 94.5 |
| Redundancy | 4.27 | 3.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 7% NaCl in 100mM Na-Acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
