4FJL

RB69 DNA polymerase ternary complex with dGTP/dA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	110
Detector technology	CCD
Collection date	2011-10-15
Detector	APEX II CCD
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	74.884, 120.511, 130.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.260 - 1.870
R-factor	0.18117
Rwork	0.179
R-free	0.22261
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.180
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.870	1.870
Number of reflections	92786
Completeness [%]	94.9	96.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, temperature 298K