4FJG

RB69 DNA polymerase ternary complex with dATP/dC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	110
Detector technology	CCD
Collection date	2011-10-15
Detector	APEX II CCD
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	75.090, 119.933, 130.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.070 - 2.020
R-factor	0.22238
Rwork	0.220
R-free	0.26383
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.224
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	2.020	2.020
Number of reflections	68757
Completeness [%]	94.3	94.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	150 mM CaCl2, 10% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate pH 6.5 , VAPOR DIFFUSION, temperature 298K