4FJC

Structure of the SAGA Ubp8/Sgf11(1-72, Delta-ZnF)/Sus1/Sgf73 DUB module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	108
Detector technology	CCD
Collection date	2011-07-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	76.490, 79.202, 265.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	265.940 - 2.826
R-factor	0.20379
Rwork	0.200
R-free	0.27587
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fip
RMSD bond length	0.011
RMSD bond angle	1.436
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	265.943	2.900
High resolution limit [Å]	2.826	2.826
Number of reflections	38300
Completeness [%]	96.8	94.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	14% PEG3350, 0.18-0.22 M tri-ammonium citrate, 8-14% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K