4FIV
FIV PROTEASE COMPLEXED WITH AN INHIBITOR LP-130
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09-02 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.650, 50.650, 74.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.149 * |
| Rwork | 0.149 |
| Structure solution method | RIGID BODY REFINEMENT |
| Starting model (for MR) | 1fiv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.046 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.092 | 0.273 |
| Number of reflections | 9993 | |
| Completeness [%] | 92.3 | 85.4 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | 4 * | pH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | DMSO | ||
| 3 | 1 | reservoir | MPD | 20-25 (%) | |
| 4 | 1 | reservoir | imidazole/HCl | 50 (mM) | |
| 5 | 1 | reservoir | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) |
