4FIB
Crystal structure of the ydhK protein from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR518A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.861, 69.493, 61.487 |
| Unit cell angles | 90.00, 113.94, 90.00 |
Refinement procedure
| Resolution | 46.486 - 1.997 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 1.997 | 2.000 |
| Rmerge | 0.154 | 0.658 |
| Number of reflections | 52101 | |
| <I/σ(I)> | 19.1 | 2.67 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 18% PEG 3350, 0.1 M monoammonium phosphate, 0.1 M MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
