4FI5

Crystal structure of the N-terminal domain of Hantaan virus strain 76-118 nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.003317
Spacegroup name	P 32 2 1
Unit cell lengths	77.400, 77.400, 36.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.1957
Rwork	0.192
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb ID 2ic9
RMSD bond length	0.014
RMSD bond angle	1.513
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.078	0.017	0.579
Number of reflections	6604	80	483
<I/σ(I)>	21.38	69.87	3.9
Completeness [%]	99.8	86	100
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	HahaA.17785.a.A16.PS01490 at 20 mg/mL against Morpheus screen condition g8, 12.5% PEG1000, 12.5% PEG3350, 12.5% MPD, 20 mM Na-formate, 20 mM Na-citrate, 20 mM Ammonium acetate, 20 mM NaK tartrate, 100 mM MOPS/HEPES-Na pH 7.5, crystal tracking ID 234641g8, puck ID hky1-5, VAPOR DIFFUSION, SITTING DROP, temperature 289K