4FHT
Crystal Structure of the PcaV transcriptional regulator from Streptomyces coelicolor in complex with its natural ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.357, 63.965, 73.009 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.651 - 2.150 |
| R-factor | 0.2018 |
| Rwork | 0.197 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nnn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.692 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 16472 | |
| <I/σ(I)> | 49.7 | 6.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.2 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 0.2 M lithium acetate, 20% w/v PEG 3350, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
