4FHQ
Crystal Structure of HVEM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 94.736, 94.736, 77.159 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.494 - 2.251 |
R-factor | 0.2796 |
Rwork | 0.279 |
R-free | 0.29250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aw2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.057 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.104 | |
Number of reflections | 8551 | |
<I/σ(I)> | 16.68 | 1.062 |
Completeness [%] | 99.3 | 94.7 |
Redundancy | 7.6 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.2 M SODIUM ACETATE TRIHYDRATE, PH 8.5, TRIS-HCL, 30% W/V PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 294.0K |