4FHM

Nup37-Nup120(aa1-961) complex from Schizosaccharomyces pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	164.053, 164.053, 310.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.497 - 4.339
R-factor	0.3035
Rwork	0.301
R-free	0.34800
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.015
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX (1.8_1063)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.419	71.419	4.550
High resolution limit [Å]	4.339	17.040	4.400
Rmerge		0.015	0.015
Total number of observations		5870	19798
Number of reflections	28523
<I/σ(I)>	8.3	13.7	0.5
Completeness [%]	99.9	97.6	99.9
Redundancy	11.8	10.5	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	10mM HEPES/NaOH pH7.5, 1.9 M MgOAc , VAPOR DIFFUSION, HANGING DROP, temperature 291.0K