4FH8
Crystal Structure of Peroxiredoxin-1 from the human hookworm Ancylostoma ceylanicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.688, 146.282, 136.030 |
| Unit cell angles | 90.00, 95.24, 90.00 |
Refinement procedure
| Resolution | 46.550 - 2.110 |
| R-factor | 0.19556 |
| Rwork | 0.195 |
| R-free | 0.21364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.949 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 2.150 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.063 | 0.643 |
| Number of reflections | 144094 | |
| <I/σ(I)> | 20.63 | 2.03 |
| Completeness [%] | 99.7 | 95.5 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 285 | 6-8% PEG3350, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
