4FGM

Crystal structure of the aminopeptidase N family protein Q5QTY1 from Idiomarina loihiensis. Northeast Structural Genomics Consortium Target IlR60.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97900
Spacegroup name	P 62 2 2
Unit cell lengths	119.657, 119.657, 220.134
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.325 - 2.394
R-factor	0.191
Rwork	0.190
R-free	0.20900
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.387
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.394	2.400
Rmerge	0.115	0.978
<I/σ(I)>	27.3	2.05
Completeness [%]	100.0	100
Redundancy	11.7	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch crystallization under oil	6.8	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5). Reservoir solution: 45% PEG/Tacsimate pH 6.8 (Hampton research HR2-092), 45% Silver Bullet (Hampton research HR2-096, G11), 0.1 M sodium citrate., Microbatch crystallization under oil, temperature 277K