4FGF

REFINEMENT OF THE STRUCTURE OF HUMAN BASIC FIBROBLAST GROWTH FACTOR AT 1.6 ANGSTROMS RESOLUTION AND ANALYSIS OF PRESUMED HEPARIN BINDING SITES BY SELENATE SUBSTITUTION

Replaces:  3FGF

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	30.900, 33.400, 35.900
Unit cell angles	59.50, 72.00, 75.60

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.161
RMSD bond length	0.016
RMSD bond angle	2.700
Refinement software	TNT

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.1 *		using macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	ammonium sulfate	2.0 (M)
2	1	2	Tris-HCl	0.1 (M)
3	1	2		0.1 (M)
4	1	2	BME	0.1 (%(v/v))