4FGB
Crystal structure of human calcium/calmodulin-dependent protein kinase I apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-10 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9640 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.046, 67.700, 56.138 |
| Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
| Resolution | 55.050 - 2.600 |
| R-factor | 0.2505 |
| Rwork | 0.248 |
| R-free | 0.29940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fg7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.056 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.050 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.121 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 88.6 | 100 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 2.0 M (NH4)2SO4, 100 mM bicine, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
