4FG6
Structure of EcCLC E148A mutant in Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Detector technology | CCD |
| Collection date | 2011-08-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 221.455, 121.284, 151.698 |
| Unit cell angles | 90.00, 128.08, 90.00 |
Refinement procedure
| Resolution | 49.551 - 3.019 |
| R-factor | 0.2389 |
| Rwork | 0.237 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ott |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.063 | 0.034 | 0.450 |
| Number of reflections | 61443 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 99.6 | 96.4 | 99.9 |
| Redundancy | 2.6 | 2.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG300, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
