4FG0
Structure of the St. Louis Encephalitis Virus envelope protein in the fusogenic trimer conformation.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 177.485, 177.485, 177.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.808 - 3.899 |
| R-factor | 0.2262 |
| Rwork | 0.222 |
| R-free | 0.26660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.520 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 4.040 |
| High resolution limit [Å] | 3.899 | 8.370 | 3.900 |
| Rmerge | 0.110 | 0.058 | 0.733 |
| Number of reflections | 8657 | ||
| <I/σ(I)> | 30.36 | 6.21 | |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 22.1 | 20.9 | 22.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop, vapor diffusion | 5.5 | 293 | 3% PEG 8000, 2% ethylene glycol, 0.1M Acetate pH 5.5, hanging drop, vapor diffusion, temperature 293K |
