4FFJ
The crystal structure of spDHBPs from S.pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.008, 78.008, 87.616 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.200 - 1.950 |
| R-factor | 0.18947 |
| Rwork | 0.187 |
| R-free | 0.24367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MERLOT |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.630 |
| High resolution limit [Å] | 1.950 | 5.880 | 2.400 |
| Rmerge | 0.070 | 0.023 | 0.176 |
| Number of reflections | 20622 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 89.5 | 89.8 | 92 |
| Redundancy | 8.8 | 8.9 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.02 | 293 | 2M NH4)2SO4,pH7.02 BisTris 0.1M, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
