4FFE

The structure of cowpox virus CPXV018 (OMCP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-12
Detector	NOIR-1
Wavelength(s)	0.9790
Spacegroup name	P 32 2 1
Unit cell lengths	105.173, 105.173, 108.657
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.334 - 2.250
R-factor	0.2161
Rwork	0.214
R-free	0.25140
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.662
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.250	4.850	2.250
Rmerge	0.050	0.031	0.486
Number of reflections	32729
<I/σ(I)>	30.86		2.73
Completeness [%]	97.8	100	88.8
Redundancy	7.7	9.6	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.9 M ammonium phosphate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.9 M ammonium phosphate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K