4FF2

N4 mini-vRNAP transcription initiation complex, 2 min after soaking GTP, ATP and Mn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-07
Detector	ADSC QUANTUM 270
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	81.908, 111.437, 275.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.674 - 2.000
R-factor	0.2082
Rwork	0.208
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.831
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.674
High resolution limit [Å]	2.000
Completeness [%]	90.3
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M TRIS-HCL, 0.21 M DIBASIC AMMONIUM CITRATE, 0.09 M AMMONIUM DIHYDROGEN MONOPHOSPHATE, 25 % (DROP) 15% (RESERVOIR) PEG 3350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K