4FEP

Crystal structure of the A24U/U25A/A46G/C74U mutant xpt-pbuX guanine riboswitch aptamer domain in complex with 2,6-diaminopurine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	133.071, 35.252, 41.855
Unit cell angles	90.00, 91.40, 90.00

Refinement procedure

Resolution	40.000 - 1.650
R-factor	0.179
Rwork	0.179
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.724
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	22255
Completeness [%]	95.7	65.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1