4FEK

Crystal Structure of putative diflavin flavoprotein A 5 (fragment 1-254) from Nostoc sp. PCC 7120, Northeast Structural Genomics Consortium Target NsR435A , Northeast Structural Genomics Consortium (NESG) Target NsR435A

Replaces:  3HNN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97921
Spacegroup name	P 1 21 1
Unit cell lengths	50.651, 57.993, 106.606
Unit cell angles	90.00, 102.42, 90.00

Refinement procedure

Resolution	28.373 - 2.000
R-factor	0.2107
Rwork	0.209
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3HNN
RMSD bond length	0.008
RMSD bond angle	1.166
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX (dev_988)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Rmerge	0.048
Number of reflections	79297
<I/σ(I)>	28.8
Completeness [%]	99.1
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl, pH 7.5. Reservoir solution:0.1 M KH2PO4, 0.1 M Tris HCL, 18% PEG3350,, VAPOR DIFFUSION, HANGING DROP, temperature 277K