4FE7
structure of xylose-binding transcription activator xylR
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95689 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.071, 70.071, 215.403 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.670 - 2.900 |
R-factor | 0.22204 |
Rwork | 0.219 |
R-free | 0.27916 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.275 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 107.830 | 107.830 | 3.060 |
High resolution limit [Å] | 2.900 | 9.170 | 2.900 |
Rmerge | 0.079 | 0.030 | 0.385 |
Number of reflections | 12704 | ||
<I/σ(I)> | 29.2 | 59.2 | 7.6 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 8.6 | 8.7 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.3 M Li2SO4 100 mM Tris pH=8.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |