4FE5
Crystal structure of the xpt-pbuX guanine riboswitch aptamer domain in complex with hypoxanthine
Replaces: 1U8DExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1271 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.247, 35.250, 41.833 |
| Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.320 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.385 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.370 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.073 | 0.467 |
| Number of reflections | 42571 | |
| <I/σ(I)> | 11.8 | 2.3 |
| Completeness [%] | 92.6 | 54.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | 25% PEG 3K, 200 mM ammonium acetate, 10 mM cobalt hexammine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
