4FE1
Improving the Accuracy of Macromolecular Structure Refinement at 7 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 281.000, 281.000, 165.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.971 - 4.923 |
| R-factor | 0.2762 |
| Rwork | 0.274 |
| R-free | 0.31450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jb0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.695 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1058)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 247.463 |
| High resolution limit [Å] | 4.900 |
| Number of reflections | 33699 |
| Completeness [%] | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.4 | 277 | beta-dodecylmaltoside, magnesium sulfate, pH 6.4, MICRODIALYSIS, temperature 277K |
