4FDZ

EutL from Clostridium perfringens, Crystallized Under Reducing Conditions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	87.970, 87.970, 252.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.824 - 1.802
R-factor	0.1609
Rwork	0.160
R-free	0.17730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4edi
RMSD bond length	0.006
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.824		1.850
High resolution limit [Å]	1.800	8.060	1.800
Rmerge	0.055	0.028	0.393
Number of reflections	91132	1070	6364
<I/σ(I)>	18.42	42.14	3.87
Completeness [%]	98.7	87.9	95
Redundancy	4.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M HEPES buffer, 5% PEG 8000, 8% ethylene glycol, 5mM tris(2-carboxyethyl)phosphine, pH 7.0, vapor diffusion, hanging drop, temperature 298K