4FDU
Crystal Structure of a Multiple Inositol Polyphosphate Phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.160, 117.300, 75.510 |
| Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
| Resolution | 37.942 - 2.293 |
| R-factor | 0.1712 |
| Rwork | 0.168 |
| R-free | 0.23070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.136 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 2.375 |
| High resolution limit [Å] | 2.290 | 2.293 |
| Number of reflections | 38461 | |
| Completeness [%] | 99.0 | 82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 289 | 0.2M Ammonium acetate pH5.0 18% PEG 3350 Protein conc.=1mg/ml, VAPOR DIFFUSION, temperature 289K |
