4FDT
Crystal Structure of a Multiple Inositol Polyphosphate Phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.720, 120.640, 76.070 |
| Unit cell angles | 90.00, 107.89, 90.00 |
Refinement procedure
| Resolution | 60.320 - 1.930 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.21310 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.976 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.070 | 1.999 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 65891 | |
| Completeness [%] | 97.1 | 82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 289 | 0.2M Ammonium acetate, pH5.0, 18% PEG 3350, Protein 1mg/ml, VAPOR DIFFUSION, temperature 289K |
