4FCM

Crystal structure of the NTF2-like domain of human G3BP1 in complex with a peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-09
Detector	MAR CCD 165 mm
Wavelength(s)	1.04
Spacegroup name	P 21 21 21
Unit cell lengths	42.780, 71.490, 89.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.380 - 2.690
R-factor	0.2277
Rwork	0.225
R-free	0.28220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fcj
RMSD bond length	0.003
RMSD bond angle	0.589
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.380	2.760
High resolution limit [Å]	2.690	2.690
Rmerge	0.129	0.943
Number of reflections	7987
<I/σ(I)>	10	1.8
Completeness [%]	99.3	99.9
Redundancy	3.9	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	279	20% PEG3350, 0.1 M Bis-tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K