4FBJ
Structure of the Cif:Nedd8 complex - Photorhabdus luminescens Cycle Inhibiting Factor in complex with human Nedd8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91340 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.730, 56.090, 67.550 |
| Unit cell angles | 90.00, 104.13, 90.00 |
Refinement procedure
| Resolution | 38.200 - 1.600 |
| R-factor | 0.16848 |
| Rwork | 0.165 |
| R-free | 0.23624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3GQJ and 1NDD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.047 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 39093 | |
| <I/σ(I)> | 11.5 | 3 |
| Completeness [%] | 96.4 | 96.2 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 289 | 20% PEG 4000, 200mM sodium acetate, 100mM MES pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
