4FBI
Crystal Structure of an R46A mutant of the Restriction-Modification Controller Protein C.Esp1396I (Trigonal Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979494 |
| Spacegroup name | P 32 |
| Unit cell lengths | 65.329, 65.329, 71.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.190 - 1.500 |
| R-factor | 0.1619 |
| Rwork | 0.160 |
| R-free | 0.19890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.889 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 71.380 | 30.186 | 1.570 |
| High resolution limit [Å] | 1.487 | 4.700 | 1.490 |
| Rmerge | 0.021 | 0.246 | |
| Total number of observations | 5067 | 19011 | |
| Number of reflections | 53279 | ||
| <I/σ(I)> | 15.3 | 23.6 | 3 |
| Completeness [%] | 95.1 | 99.3 | 97 |
| Redundancy | 2.4 | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 0.1M MIB buffer, 25% w/v PEG 1500, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
