4FBD
2.35 Angstrom Crystal Structure of Conserved Hypothetical Protein from Toxoplasma gondii ME49.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.599, 87.216, 112.122 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.400 - 2.350 |
| R-factor | 0.20509 |
| Rwork | 0.202 |
| R-free | 0.25826 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pd0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.471 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.066 | 0.557 |
| Number of reflections | 20165 | |
| <I/σ(I)> | 27.6 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | Protein: 7.0mg/mL, 0.5M Sodium cloride, Tris-HCl pH 8.3; Screen: JCSG+ (F11), 1M Succinic acid pH 7.0, 0.1M HEPES pH 7.0, 1.0% (w/v) PEG MME 2000; Cryo: Screen solution : 50% Sucrose, 1:1 (v/v), VAPOR DIFFUSION, SITTING DROP, temperature 295K |
