4FB7
The apo form of idole-3-glycerol phosphate synthase (TrpC) form Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.638, 58.504, 80.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.260 - 1.300 |
| R-factor | 0.1349 |
| Rwork | 0.135 |
| R-free | 0.15360 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.526 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 3.530 | 1.300 |
| Rmerge | 0.120 | 0.068 | 0.619 |
| Number of reflections | 65545 | ||
| <I/σ(I)> | 21.5 | 2.38 | |
| Completeness [%] | 96.4 | 99.8 | 91.6 |
| Redundancy | 7.8 | 8.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M Bis-Tris/HCl, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
