4FB3

Polyomavirus T-ag binds symmetrical repeats at the viral origin in an asymmetrical manner

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	C 2 2 21
Unit cell lengths	165.150, 167.922, 77.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.931 - 3.790
R-factor	0.2229
Rwork	0.222
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.035
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall
Low resolution limit [Å]	117.460
High resolution limit [Å]	3.790
Number of reflections	11084
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	277	14% PEG 3350, 140 mM sodium citrate, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K