4FB0
Structure of Oceanobacillus iheyensis group II intron C377G mutant in a ligand-free state in the presence of K+ and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.188, 95.444, 224.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.160 - 3.220 |
| R-factor | 0.18608 |
| Rwork | 0.184 |
| R-free | 0.22958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3igi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.587 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.066 | 1.023 |
| Number of reflections | 30139 | |
| <I/σ(I)> | 15.6 | 1.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 100 mM magnesium acetate, 150 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 5% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
