4FAZ
Kinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-08-21 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.170, 76.169, 69.876 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.220 - 1.570 |
R-factor | 0.23103 |
Rwork | 0.229 |
R-free | 0.27466 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bjp |
RMSD bond length | 0.021 |
RMSD bond angle | 2.149 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.220 | 1.610 |
High resolution limit [Å] | 1.570 | 1.570 |
Number of reflections | 20889 | |
Completeness [%] | 98.5 | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 200 mM tri-Ammonium Citrate, 10% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |