4FAS
Complex crystal structure of hydroxylamine oxidoreductase and NE1300 from Nitrosomonas europaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 140.731, 142.618, 107.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.890 - 2.100 |
| R-factor | 0.16701 |
| Rwork | 0.165 |
| R-free | 0.20583 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Monomer of PDB ID: 1FGJ |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.127 | 0.574 |
| Number of reflections | 109103 | |
| <I/σ(I)> | 9.554 | 2.036 |
| Completeness [%] | 86.7 | 69.3 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 292 | 0.1 M potassium nitrate, 0.1 M MES pH 7.5 and 46%(v/v) PEG 400, VAPOR DIFFUSION, temperature 292K |
