4FAA
Structure of Recombinant Cytochrome ba3 Oxidase mutant A120F+A204F from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.280, 98.290, 95.050 |
| Unit cell angles | 90.00, 127.50, 90.00 |
Refinement procedure
| Resolution | 56.836 - 2.800 |
| R-factor | 0.1968 |
| Rwork | 0.194 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.151 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 2011_01_25_1225)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.836 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 25136 |
| Completeness [%] | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 50mM Sodium Cacodylate, 1.6M NaCl, 40% PEG400, pH 6.5, Lipidic Cubic Phase, temperature 293K |
