4F9Z

Crystal Structure of human ERp27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-23
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 1
Unit cell lengths	57.832, 68.359, 86.775
Unit cell angles	70.45, 88.16, 64.96

Refinement procedure

Resolution	40.675 - 2.200
R-factor	0.171
Rwork	0.170
R-free	0.21690
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.070
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.810	57.810	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.178
Number of reflections	72979
<I/σ(I)>	8.8
Completeness [%]	95.9	97.1	95.1
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% PEG 4000, 150mM sodium acetate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
2	VAPOR DIFFUSION, HANGING DROP	8.5	293	26% PEG 4000, 125mM sodium acetate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K