4F9N
Crystal structure of the complex of type I Ribosome inactivating protein from Momordica balsamina with N7-methylated guanine at 2.65 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 77 |
Detector technology | IMAGE PLATE |
Collection date | 2009-07-21 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | H 3 |
Unit cell lengths | 131.428, 131.428, 39.808 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.610 - 2.650 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s9q |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.760 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.100 | 0.586 |
Number of reflections | 7364 | |
<I/σ(I)> | 9.3 | 2 |
Completeness [%] | 99.3 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |