4F9K

Crystal Structure of human cAMP-dependent protein kinase type I-beta regulatory subunit (fragment 11-73), Northeast Structural Genomics Consortium (NESG) Target HR8613A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-24
Detector	MAR CCD 165 mm
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	82.270, 50.070, 75.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.300 - 2.800
R-factor	0.257
Rwork	0.254
R-free	0.32400
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.475
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (dev_988)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Rmerge	0.056
Number of reflections	13955
<I/σ(I)>	14.5
Completeness [%]	93.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch under oil	6.5	291	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution:0.2M (NH4)2SO4, 0.1M Na-c, microbatch under oil, temperature 291K