4F9F

Crystal Structure of VldE, the pseudo-glycosyltransferase, in complex with GDP and Trehalose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	320.821, 122.823, 93.857
Unit cell angles	90.00, 91.62, 90.00

Refinement procedure

Resolution	49.153 - 2.807
R-factor	0.2136
Rwork	0.211
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.466
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.153	2.960
High resolution limit [Å]	2.807	2.807
Number of reflections	88243
Completeness [%]	99.3	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	50 mM Tris-HCl, pH 8.0, 18-28% PEG3350, 200-500 mM sodium chloride, 20 mM magnesium chloride, 3% trehalose, 1 mM GDP, VAPOR DIFFUSION, SITTING DROP, temperature 293K